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Bio-Rad
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Oxford Nanopore
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Image Search Results
Journal: Developmental cell
Article Title: Long-range optogenetic control of axon guidance overcomes developmental boundaries and defects
doi: 10.1016/j.devcel.2020.05.009
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-synaptic vesicle 2 (Clone SV2) DSHB Cat#SV2; RRID: AB_2315387 Rabbit polyclonal anti-RFP Rockland Cat#600-401-379S Lot 35868; RRID: AB_2209751 Rabbit polyclonal anti-Thr423 phospho-PAK1 Cell Signaling Technology Cat#2601T; RRID: AB_330220 Rat monoclonal anti-RFP (Clone 5F8) ChromoTek Cat#5F8; RRID: AB_2336064 AlexaFluor 488 conjugated goat anti-mouse Invitrogen Cat#A11001; RRID: AB_2534069 AlexaFluor 488 conjugated goat anti-rabbit Invitrogen Cat#A11008; RRID: AB_143165 AlexaFluor 546 conjugated goat anti-rabbit Invitrogen Cat#A11035; RRID: AB_143051 AlexaFluor 546 conjugated got anti-rat Invitrogen Cat#A11081; RRID: AB_141738 Chemicals, Peptides, and Recombinant Proteins Leibovitz’s L-15 media Thermo Fisher Cat#2108027 Alpha-bungarotoxin, Alexa Fluor 647 conjugate Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"B35450","term_id":"2534819","term_text":"B35450"}} B35450 LR Clonase II Plus Invitrogen Cat#12538200 Quick T4 DNA ligase Neb Cat#M2200S Poly-D-Lysine VWR Cat#35210 Laminin VWR Cat#354232 Ethyl 3-aminobenzoate (Tricaine, MS222) Sigma-Aldrich Cat# {"type":"entrez-nucleotide","attrs":{"text":"E10505","term_id":"22027338","term_text":"E10505"}} E10505 Agarose type II (low melting point) Sigma-Aldrich Cat#A6877 Trizol LS Thermo Fisher Cat#10296010 Collagenase Sigma-Aldrich Cat#C9891 Critical Commercial Assays DNAeasy Blood and Tissue Kit Qiagen 69504 Custom TaqMan SNP Genotyping Assay Thermo Fischer Cat#4332077; AssayID: ANXGU9F pENTR 5’-TOPO TA-Cloning Kit Invitrogen Cat#K59120 pENTR/D-TOPO cloning kit Invitrogen Cat#K240020 Q5 site directed mutagenesis kit NEB Cat#E0554S Gibson Assembly kit NEB Cat#E5510S
Techniques: Recombinant, TaqMan SNP Genotyping Assay, Clone Assay, Mutagenesis, Construct, Plasmid Preparation, Software, Microscopy
Journal: mAbs
Article Title: NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cells
doi: 10.1080/19420862.2022.2106621
Figure Lengend Snippet: Summary of antibody gene sequencing using NAb-seq.
Article Snippet: cDNA amplification and barcoding , Oxford Nanopore PCR-cDNA Barcoding kit (SQK-PCB109) , , NEBNext® single cell/low input cDNA synthesis & amplification module, NEBNext Ultra II end repair/dA-tailing module, Oxford Nanopore Native Barcoding Expansion (PCR-free) kit (EXP-NBD114),
Techniques: Sequencing, cDNA Library Assay, Amplification, cDNA Synthesis, Adapter Ligation, Ligation, Generated